How Diclofenac Affects Antibiotic Resistance in Staphylococcus aureus
Author Information
Author(s): Riordan James T, Dupre JoAnne M, Cantore-Matyi Stephanie A, Kumar-Singh Atul, Song Yang, Zaman Shahrear, Horan Sonia, Helal Nada S, Nagarajan Vijayaraj, Elasri Mohamed O, Wilkinson Brian J, Gustafson John E
Primary Institution: University of South Florida
Hypothesis
Diclofenac alters the transcriptome and antibiotic susceptibility of Staphylococcus aureus.
Conclusion
Diclofenac influences antibiotic susceptibility in Staphylococcus aureus by altering the expression of genes related to cell wall biosynthesis and transport.
Supporting Evidence
- Diclofenac significantly altered the expression of 458 genes in S. aureus.
- Susceptibility to fluoroquinolone antibiotics increased in the presence of diclofenac.
- Diclofenac reduced the expression of genes associated with antibiotic resistance mechanisms.
Takeaway
Diclofenac, a common pain reliever, can change how bacteria respond to antibiotics, making some antibiotics work better and others less effective.
Methodology
The study used gene expression microarrays and quantitative real-time PCR to analyze transcriptome changes and antibiotic susceptibility tests to measure the effects of diclofenac.
Limitations
The study primarily focused on specific strains of Staphylococcus aureus and may not generalize to all strains or conditions.
Statistical Information
P-Value
0.01
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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