Molecular identification of Coccidioides spp. in soil samples from Brazil
2011

Identifying Coccidioides in Brazilian Soil Samples

Sample size: 24 publication Evidence: high

Author Information

Author(s): Regina CL de Macêdo, Alexandre S Rosado, Fabio F da Mota, Maria AS Cavalcante, Kelsen D Eulálio, Antônio D Filho, Liline MS Martins, Márcia S Lazéra, Bodo Wanke

Primary Institution: Instituto de Pesquisa Clínica Evandro Chagas, Fundação Oswaldo Cruz, Rio de Janeiro, Brasil

Hypothesis

Can molecular methods effectively identify Coccidioides spp. in soil samples from Brazil?

Conclusion

The study developed a sensitive and specific molecular technique for detecting Coccidioides spp. in soil, although it cannot distinguish between species.

Supporting Evidence

  • 24 soil samples were collected from areas suspected of contamination.
  • 6 out of 24 samples tested positive for C. posadasii using animal inoculation.
  • All samples were positive for Coccidioides spp. using molecular methods.

Takeaway

Researchers found a way to detect a fungus in the soil that can make people sick, using a special test that works really well.

Methodology

Soil samples were collected and analyzed using both animal inoculation and molecular techniques, including semi-nested PCR.

Limitations

The methodology cannot distinguish between C. immitis and C. posadasii and may miss viable elements in soil.

Digital Object Identifier (DOI)

10.1186/1471-2180-11-108

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