Detection of CD133+ Cancer Stem Cells in Osteosarcoma
Author Information
Author(s): Tirino Virginia, Desiderio Vincenzo, d'Aquino Riccardo, De Francesco Francesco, Pirozzi Giuseppe, Galderisi Umberto, Cavaliere Carlo, De Rosa Alfredo, Papaccio Gianpaolo
Primary Institution: Dipartimento di Medicina Sperimentale, Seconda Università degli Studi di Napoli, Napoli, Italy
Hypothesis
Can CD133 antigen be used to identify cancer stem cells in osteosarcoma cell lines?
Conclusion
The study identifies CD133+ cells in osteosarcoma cell lines, suggesting their role as cancer stem cells.
Supporting Evidence
- CD133+ cells were identified in all three osteosarcoma cell lines tested.
- CD133+ cells showed a high proliferation rate and the ability to form sphere clusters.
- The study demonstrated that CD133+ cells are capable of extensive proliferation compared to CD133− cells.
Takeaway
The researchers found special cells in bone cancer that can grow and multiply quickly, which might help in creating better treatments.
Methodology
The study used flow cytometry to identify CD133+ cells in osteosarcoma cell lines and assessed their proliferation and tumorigenicity.
Limitations
In vivo experiments could not be performed due to the inability of osteosarcoma cell lines to graft in hosts.
Participant Demographics
Cell lines isolated from young Caucasian subjects.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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