Protocol for Preparing Alveolar Epithelial Type I Cells from Mouse Lungs
Author Information
Author(s): Huang Lulu, Tang Amy, Shao Bojing
Primary Institution: Lindsley F. Kimball Research Institute, New York Blood Center
Conclusion
This protocol allows for the direct preparation of primary alveolar epithelial type I cells from mouse lungs with a high purity and yield.
Supporting Evidence
- The protocol yields 1 to 3 million AT1 cells per mouse.
- The purity of AT1 cells is assessed to be 89.47 ± 5.08%.
- The protocol reduces the heterogeneity seen in AT1 cells derived from AT2 cells.
Takeaway
Scientists can now easily get special lung cells from mice without needing to change other cells first, which helps them study lung diseases better.
Methodology
The protocol involves dissociating epithelial cells from mouse lungs, purifying AT1 cells using magnetic-activated cell sorting, and characterizing their purity through immunofluorescence staining and flow cytometry.
Limitations
The lack of self-renewal ability of AT1 cells limits their use in studies to approximately two weeks.
Participant Demographics
C57BL/6J mice, both males and females, aged 4–6 months.
Statistical Information
Confidence Interval
89.47 ± 5.08%
Digital Object Identifier (DOI)
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