Gene Expression Analysis of Lobular Carcinoma in Situ
Author Information
Author(s): Cao Dengfeng, Polyak Kornelia, Halushka Marc K, Nassar Hind, Kouprina Nina, Iacobuzio-Donahue Christine, Wu Xinyan, Sukumar Saraswati, Hicks Jessica, De Marzo Angelo, Argani Pedram
Primary Institution: Washington University, Dana Farber Cancer Institute, Johns Hopkins Medical Institutions
Hypothesis
Lobular carcinoma in situ (LCIS) is a low-risk precursor to invasive lobular carcinoma, and its gene expression profile can reveal important molecular alterations.
Conclusion
The study identified down regulation of claudin 4 and overexpression of matrix metalloproteinase 9 in lobular carcinoma in situ, suggesting potential therapeutic targets.
Supporting Evidence
- Claudin 4 was found to be down regulated in lobular carcinoma in situ compared to normal breast epithelium.
- Matrix metalloproteinase 9 was overexpressed in lobular carcinoma in situ relative to normal breast epithelium.
- Immunohistochemistry confirmed the findings of gene expression analysis.
- RNA analysis showed that only MMP9 was overexpressed in lobular carcinoma in situ.
Takeaway
Researchers looked at a type of breast cancer called lobular carcinoma in situ and found that it has some important changes in its genes that could help doctors treat it better.
Methodology
The study used serial analysis of gene expression (SAGE) to analyze the gene expression profile of lobular carcinoma in situ and validated findings through immunohistochemistry and quantitative RT-PCR.
Limitations
The analysis was based on a single case of lobular carcinoma in situ with unusual features, which may limit the generalizability of the findings.
Participant Demographics
The study involved a post-pubertal, pre-menopausal female patient.
Statistical Information
P-Value
0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website