Effects of Bacterial Lipopolysaccharide on Trout Ovary
Author Information
Author(s): Simon MacKenzie, Nuria Montserrat, Mas Mario, Acerete Laura, Tort Lluis, Krasnov Aleksei, Goetz Frederick W, Planas Josep V
Primary Institution: Universitat de Barcelona
Hypothesis
How does lipopolysaccharide (LPS) affect the reproductive function of sexually mature female trout?
Conclusion
LPS administration in trout did not impair ovarian hormone production or oocyte maturation but significantly increased apoptosis in follicular cells.
Supporting Evidence
- LPS treatment did not affect plasma testosterone concentration.
- LPS increased plasma cortisol concentration.
- LPS administration in vivo induced apoptosis in follicular cells.
- LPS did not impair oocyte maturation in trout.
- LPS did not affect the basal or LH-stimulated steroid production in vitro.
Takeaway
When trout were given a substance from bacteria, it didn't stop their eggs from maturing, but it did cause some cells in their ovaries to die.
Methodology
Brook trout were injected with saline or LPS, and various assays were conducted to measure steroid production, oocyte maturation, and apoptosis.
Limitations
The study only assessed short-term effects of LPS administration and did not evaluate long-term impacts on ovarian function.
Participant Demographics
Sexually mature female brook trout (Salvelinus fontinalis) and rainbow trout (Oncorhynchus mykiss).
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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