Proteomic Analysis of Aedes albopictus Cells Infected with Dengue Viruses
Author Information
Author(s): Patramool Sirilaksana, Surasombatpattana Pornapat, Luplertlop Natthanej, Sévéno Martial, Choumet Valérie, Thomas Frédéric, Missé Dorothée
Primary Institution: Laboratoire Maladies Infectieuses et Vecteurs: Ecologie, Génétique, Evolution, Contrôle., UMR 5290 CNRS/IRD/UM1, Montpellier, France
Hypothesis
What proteins are regulated during infection by Dengue serotypes 1 and 3 in an Aedes albopictus cell line?
Conclusion
The study found that infection with Dengue viruses leads to significant changes in protein expression related to stress response and glycolysis in mosquito cells.
Supporting Evidence
- The study identified 22 differentially expressed protein spots in infected cells.
- Five enzymes involved in glycolysis were significantly up-regulated in virus-infected cells.
- Viral infection activated the translation of host genes related to stress response.
Takeaway
When mosquitoes get sick from Dengue viruses, their cells change how they work to try to fight off the virus and stay alive.
Methodology
The study used two-dimensional differential in-gel electrophoresis (2D-DIGE) to compare the proteomes of infected and non-infected Aedes albopictus cells.
Limitations
The study focused only on two serotypes of Dengue virus and one cell line, which may limit the generalizability of the findings.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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