Olfactory Interference during Inhibitory Backward Pairing in Honey Bees
2008

Olfactory Interference in Honey Bees

publication 10 minutes Evidence: moderate

Author Information

Author(s): Dacher Matthieu, Smith Brian H.

Primary Institution: School of Life Sciences, Arizona State University, Tempe, Arizona, United States of America

Hypothesis

Does backward pairing of odor and sucrose affect the proboscis extension response in honey bees?

Conclusion

Olfactory interference occurs when an odor is presented 15 seconds after sucrose feeding, leading to a reduction in the proboscis extension response.

Supporting Evidence

  • Backward pairing of odor and sucrose leads to inhibitory learning in honey bees.
  • Olfactory interference was observed specifically at a 15-second delay after sucrose feeding.
  • Injection of cimetidine impaired the olfactory interference effect.
  • Bees that received odor stimulation showed a significant decline in proboscis extension response.

Takeaway

When bees eat sugar and then smell an odor 15 seconds later, they stop extending their tongue to get more sugar. This shows how smells can change their behavior.

Methodology

Bees were fed sucrose and then exposed to an odor at different time intervals to observe changes in their proboscis extension response.

Limitations

The study primarily focused on a specific time interval (15 seconds) and may not generalize to other time frames.

Participant Demographics

Worker honey bees (Apis mellifera) were used in the experiments.

Statistical Information

P-Value

p=0.004

Confidence Interval

95% confidence interval for survival rates

Statistical Significance

p<0.05

Digital Object Identifier (DOI)

10.1371/journal.pone.0003513

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