Boldenone and Testosterone Production from Phytosterol via One-Pot Cascade Biotransformations
2024

Producing Testosterone and Boldenone from Phytosterol Using Fungi

publication Evidence: high

Author Information

Author(s): Vyacheslav V. Kollerov, Tatiana A. Timakova, Andrei A. Shutov, Marina V. Donova, Edvaldo Antonio Ribeiro Rosa, Sanjai Saxena

Primary Institution: Federal Research Center, Pushchino Center for Biological Research of Russian Academy of Sciences

Hypothesis

Can the ascomycete Curvularia sp. efficiently convert phytosterol into testosterone and boldenone through cascade biotransformations?

Conclusion

The study demonstrates that Curvularia sp. can effectively convert phytosterol into testosterone and boldenone, showcasing its biotechnological potential.

Supporting Evidence

  • Curvularia sp. was shown to convert androstadienedione to yield 97% boldenone.
  • The study developed a novel cascade biotransformation process for producing testosterone and boldenone from phytosterol.
  • Six candidate genes encoding 17β-hydroxysteroid dehydrogenases were identified in the Curvularia sp. genome.

Takeaway

Scientists found a way to make important medicines from plants using a special fungus, which is good for the environment.

Methodology

The study involved using the fungus Curvularia sp. in combination with Mycolicibacterium strains to convert phytosterol into testosterone and boldenone through a two-step biotransformation process.

Limitations

The study did not explore the economic feasibility of the biotransformation process on a larger scale.

Digital Object Identifier (DOI)

10.3390/jof10120830

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