Study of Squid and Bacteria Interaction
Author Information
Author(s): Chun Carlene K, Scheetz Todd E, Bonaldo Maria de Fatima, Brown Bartley, Clemens Anik, Crookes-Goodson Wendy J, Crouch Keith, DeMartini Tad, Eyestone Mari, Goodson Michael S, Janssens Bernadette, Kimbell Jennifer L, Koropatnick Tanya A, Kucaba Tamara, Smith Christina, Stewart Jennifer J, Tong Deyan, Troll Joshua V, Webster Sarahrose, Winhall-Rice Jane, Yap Cory, Casavant Thomas L, McFall-Ngai Margaret J, Soares M Bento
Primary Institution: University of Wisconsin-Madison
Hypothesis
How do the bacterial partners impact one another's developmental programs?
Conclusion
The study generated a significant number of expressed sequence tags (ESTs) that reflect the activity state of the squid light organ during early symbiosis with Vibrio fischeri.
Supporting Evidence
- Generated 42,564 expressed sequence tags (ESTs) from the squid light organ.
- 35,421 ESTs passed quality criteria and were clustered into 13,962 nonredundant sequences.
- 70% of the nonredundant ESTs had significant hits to proteins in the NCBI database.
Takeaway
The researchers looked at how a squid and its bacteria work together, finding many important genes that help them grow and develop together.
Methodology
The study involved generating 11 cDNA libraries from the light organ of juvenile squid at different developmental stages, followed by sequencing and analysis of expressed sequence tags.
Limitations
The study may not capture all aspects of the squid-bacteria interaction due to the focus on specific developmental time points.
Participant Demographics
Juvenile Euprymna scolopes squid.
Digital Object Identifier (DOI)
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