Effects of Two Natural Compounds on STAT3 Activation
Author Information
Author(s): Butturini Elena, Cavalieri Elisabetta, Carcereri de Prati Alessandra, Darra Elena, Rigo Antonella, Shoji Kazuo, Murayama Norie, Yamazaki Hiroshi, Watanabe Yasuo, Suzuki Hisanori, Mariotto Sofia
Primary Institution: Department of Life and Reproduction Sciences, University of Verona, Verona, Italy
Hypothesis
Dehydrocostuslactone (DCE) and costunolide (CS) inhibit STAT3 activation by decreasing intracellular GSH levels.
Conclusion
DCE and CS inhibit STAT3 activation through a decrease in intracellular GSH levels and subsequent S-glutathionylation of STAT3.
Supporting Evidence
- DCE and CS inhibit IL-6-elicited STAT3 activation in THP-1 cells.
- The EC50 for the inhibition of STAT3 activation by DCE and CS is 10 µM.
- DCE and CS induce a drop in intracellular GSH levels.
- Glutathione monoethyl ester (GEE) can reverse the inhibitory action of DCE and CS on STAT3.
- DCE and CS induce S-glutathionylation of STAT3.
Takeaway
Two natural compounds can help stop a protein called STAT3 from working too much by lowering a substance called GSH in cells.
Methodology
The study used human THP-1 cells to assess the effects of DCE and CS on STAT3 activation and intracellular GSH levels through various biochemical assays.
Limitations
The study primarily focused on in vitro experiments, which may not fully represent in vivo conditions.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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