Structural and functional basis for RNA cleavage by Ire1
2011
How Ire1 Cleaves RNA: Understanding the Mechanism
publication
Evidence: high
Author Information
Author(s): Korennykh Alexei V, Korostelev Andrei A, Egea Pascal F, Finer-Moore Janet, Stroud Robert M, Zhang Chao, Shokat Kevan M, Walter Peter
Primary Institution: Howard Hughes Medical Institute, University Of California, San Francisco
Hypothesis
What is the mechanism of RNA cleavage by Ire1?
Conclusion
The study defines the catalytic center of Ire1 RNase and suggests a mechanism for RNA cleavage involving specific residues.
Supporting Evidence
- Ire1 RNase uses specific residues for RNA cleavage.
- Histidine H1061 is crucial for catalysis.
- Ire1 can cleave tRNAPhe, expanding its known substrate specificity.
Takeaway
Ire1 is like a pair of scissors for RNA, and this study shows how it cuts RNA using special parts of its structure.
Methodology
The study combined quantitative analysis of Ire1 mutants and X-ray crystallography to understand RNA cleavage.
Digital Object Identifier (DOI)
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