The fission yeast DNA structure checkpoint protein Rad26ATRIP/LCD1/UVSD accumulates in the cytoplasm following microtubule destabilization
2006
Rad26 and Rad24 in Fission Yeast Respond to Microtubule Destabilization
publication
Evidence: moderate
Author Information
Author(s): Baschal Erin E, Chen Kuan J, Elliott Lee G, Herring Matthew J, Verde Shawn C, Wolkow Tom D
Primary Institution: University of Colorado at Colorado Springs
Hypothesis
How do Rad26 and Rad24 proteins respond to microtubule destabilizing conditions in fission yeast?
Conclusion
Rad26 and Rad24 are crucial for responding to microtubule destabilizing conditions in fission yeast.
Supporting Evidence
- Rad26-GFP accumulates in the cytoplasm following treatment with microtubule destabilizing drugs.
- Loss of rad26+ causes sensitivity to microtubule destabilizing agents.
- Rad24 is required for the cytoplasmic accumulation of Rad26-GFP during microtubule destabilization.
Takeaway
This study shows that two proteins, Rad26 and Rad24, help yeast cells deal with problems caused by drugs that disrupt their microtubules, which are important for cell shape and division.
Methodology
The study involved experiments with fission yeast strains, including growth assays and microscopy to observe protein localization.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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