Hysteretic Behavior of Proprotein Convertase 1/3 (PC1/3)
Author Information
Author(s): Icimoto Marcelo Y., Barros Nilana M., Ferreira Juliana C., Marcondes Marcelo F., Andrade Douglas, Machado Mauricio F., Juliano Maria A., Júdice Wagner A., Juliano Luiz, Oliveira Vitor
Primary Institution: Universidade Federal de São Paulo, São Paulo, Brazil
Hypothesis
The study aims to explore the unusual lag phase observed in the activity of mouse proprotein convertase 1/3 (mPC1/3).
Conclusion
The study demonstrates that mPC1/3 exhibits a lag phase of activation that is dependent on substrate, calcium concentration, and pH, indicating a hysteretic behavior.
Supporting Evidence
- mPC1/3 has a lag phase of activation that can be interpreted as hysteretic behavior.
- The lag phase is dependent on substrate, calcium concentration, and pH.
- This hysteretic behavior may play a role in physiological processes involving PC1/3.
Takeaway
This study found that an enzyme called PC1/3 takes some time to start working when it gets a new job, and this delay can change based on what it's working with.
Methodology
The study involved enzyme preparation, kinetic analysis of substrate hydrolysis, and examination of the effects of calcium and pH on enzyme activity.
Limitations
The study was limited to specific substrate concentrations and pH ranges, which may not encompass all physiological conditions.
Digital Object Identifier (DOI)
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