Cross-Species Gene Expression Analysis in Mammals
Author Information
Author(s): Manuel Nieto-Díaz, Wolfgang Pita-Thomas, Manuel Nieto-Sampedro
Primary Institution: Instituto de Neurobiología Ramón y Cajal (CSIC)
Hypothesis
Are hybridization values from cross-species analyses as reproducible as those from same-species analyses when using Affymetrix oligonucleotide microarrays?
Conclusion
Cross-species analyses do not significantly affect hybridization reproducibility of GeneChips for the mammal species analyzed.
Supporting Evidence
- The study found that cross-species hybridization did not affect the distribution of hybridization reproducibility.
- Only 0.5% of the probes analyzed were significantly associated with un-reproducible hybridizations.
- The results suggest that previous differences in reproducibility were likely due to analytical methods rather than hybridization issues.
Takeaway
This study shows that using gene expression data from different mammal species on the same microarray is reliable and gives consistent results.
Methodology
The study compared hybridization reproducibility of RNA samples from deer, Old-World primates, and humans using Affymetrix GeneChip U133 Plus 2.0.
Limitations
The study is limited to the range of mammal species analyzed and the specific microarray technology used.
Participant Demographics
RNA samples from deer and Old-World primates were used.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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