Genome-wide study of genetic changes in esophageal cancer
Author Information
Author(s): Hu Nan, Wang Chaoyu, Hu Ying, Yang Howard H, Kong Li-Hui, Lu Ning, Su Hua, Wang Quan-Hong, Goldstein Alisa M, Buetow Kenneth H, Emmert-Buck Michael R, Taylor Philip R, Lee Maxwell P
Primary Institution: Genetic Epidemiology Branch, Division of Cancer Epidemiology and Genetics, NCI, USA
Hypothesis
Can genome-wide detection of chromosomal changes improve understanding of esophageal squamous cell carcinoma?
Conclusion
The study shows that the Affymetrix 10 K SNP chip is effective for analyzing loss of heterozygosity and copy number alterations in esophageal squamous cell carcinoma.
Supporting Evidence
- Non-random loss of heterozygosity was found on 10 chromosomal arms.
- Twenty novel loss of heterozygosity regions were identified.
- Fifteen regions of copy number loss and 36 regions of copy number gain were detected.
Takeaway
Researchers looked at DNA from 26 patients with esophageal cancer to find changes that might help understand the disease better.
Methodology
The study used the Affymetrix 10 K SNP array to analyze matched germ-line and tumor DNA samples for loss of heterozygosity and copy number alterations.
Limitations
The study's findings may not be generalizable beyond the specific high-risk population in Shanxi, China.
Participant Demographics
Patients diagnosed with esophageal squamous cell carcinoma in Shanxi Province, China, between 1998 and 2000.
Statistical Information
P-Value
p ≤ 10-6
Statistical Significance
p ≤ 10-6
Digital Object Identifier (DOI)
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