RNA Oxidation Changes in Alzheimer's Disease
Author Information
Author(s): Weidner Adam M., Bradley Melissa A., Beckett Tina L., Niedowicz Dana M., Dowling Amy L. S., Matveev Sergey V., LeVine Harry III, Lovell Mark A., Murphy M. Paul
Primary Institution: University of Kentucky
Hypothesis
What is the relationship between RNA oxidation and amyloid-β levels in late-stage Alzheimer's disease?
Conclusion
The study found that while RNA oxidation is connected to Alzheimer's disease pathology, the relationship is complex and not straightforward.
Supporting Evidence
- RNA adduct 8-OHG decreased in Alzheimer's disease.
- RNA adduct 8-OHA increased in Alzheimer's disease.
- No RNA adducts correlated with the number of plaques or tangles.
- SDS-soluble Aβ42 was the best predictor of changes in 8-OHG.
- Formic acid-soluble Aβ42 was the best predictor of changes in 8-OHA.
Takeaway
In Alzheimer's disease, some types of RNA damage go up while others go down, and this is linked to different forms of a protein called amyloid-β.
Methodology
The study quantified five RNA adducts using gas chromatography/mass spectrometry across brain regions from Alzheimer's disease cases and age-matched controls.
Limitations
The study only included late-stage Alzheimer's cases and did not assess earlier stages of the disease.
Participant Demographics
12 Alzheimer's disease cases and 10 age-matched controls, with a mix of genders.
Statistical Information
P-Value
p=0.046 for 8-OHG, p=0.038 for 8-OHA
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website