HPLC Method for Analyzing Cannabinoids in Veterinary CBD Oil
Author Information
Author(s): Hajrulai-Musliu Zehra, Dimitreska Stojkovikj Elizabeta, Gusheski Dimitar, Musliu Dea, Velkovski Daniel
Primary Institution: Faculty of Veterinary Medicine-Skopje, University “Ss. Cyril and Methodius”
Hypothesis
Can a validated HPLC method accurately identify and quantify cannabinoids in commercial veterinary CBD oil?
Conclusion
The study developed a reliable HPLC method for analyzing cannabinoids in CBD oil, confirming that product cannabinoid levels matched manufacturers' claims.
Supporting Evidence
- The method was validated for linearity, accuracy, and precision.
- All analyzed products had cannabinoid levels consistent with the manufacturers' declared specifications.
- The method exhibited acceptable precision with relative standard deviation values lower than or equal to 2%.
- The limit of detection for cannabinoids ranged from 0.05 to 0.13 µg/mL.
- The study highlights the importance of quality control in CBD oil products.
Takeaway
This study shows how scientists can use a special test to check if the amount of CBD in pet oils is what the labels say.
Methodology
The study used high-performance liquid chromatography (HPLC) with diode array detection to analyze cannabinoid content in CBD oil.
Limitations
The study only analyzed 14 products, which may not represent the entire market.
Digital Object Identifier (DOI)
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