Identification of angiogenic properties of insulin-like growth factor II in in vitro angiogenesis models
2000
How Insulin-like Growth Factor II Helps Blood Vessel Formation
publication
Evidence: moderate
Author Information
Author(s): Lee O H, Bae S K, Bae M H, Lee Y M, Moon E J, Cha H J, Kwon Y G, Kim K W
Primary Institution: Pusan National University
Hypothesis
Does insulin-like growth factor II (IGF-II) promote angiogenesis in vitro?
Conclusion
The study found that IGF-II directly induces angiogenesis by stimulating the migration and differentiation of endothelial cells.
Supporting Evidence
- IGF-II treatment caused HUVECs to align and form a capillary-like network.
- IGF-II significantly increased the migration of HUVECs in chemotaxis assays.
- Activation of p38 MAPK and p125FAK was observed in HUVECs exposed to IGF-II.
- IGF-II stimulated HUVEC invasion through Matrigel-coated filters.
- MMP-2 activity in HUVECs was increased by IGF-II, correlating with increased MMP-2 mRNA expression.
Takeaway
Insulin-like growth factor II helps cells that form blood vessels move and change shape, which is important for growing new blood vessels.
Methodology
The study used human umbilical vein endothelial cells (HUVECs) in various assays to assess the effects of IGF-II on angiogenesis.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website