New Tests for Angiopoietins in Critically Ill Patients
Author Information
Author(s): Lukasz Alexander, Hellpap Julian, Horn Rüdiger, Kielstein Jan T, David Sascha, Haller Hermann, Kümpers Philipp
Primary Institution: Hannover Medical School
Hypothesis
Can circulating Ang-1 and Ang-2 serve as reliable biomarkers for endothelial integrity in critically ill patients?
Conclusion
Ang-1 and Ang-2 might serve as a novel class of biomarker in critically ill patients.
Supporting Evidence
- Ang-1 and Ang-2 concentrations correlated with severity of illness in critically ill patients.
- The assays had detection limits of 0.12 ng/ml for Ang-1 and 0.2 ng/ml for Ang-2.
- Ang-1 and Ang-2 concentrations were stable for 24 hours and resistant to freeze-thaw cycles.
Takeaway
Doctors can use new tests to measure two important proteins, Ang-1 and Ang-2, in sick patients to see how well their blood vessels are working.
Methodology
Developed sandwich immunoassays for Ang-1 and Ang-2, tested on 30 healthy controls and 94 critically ill patients.
Limitations
The study did not focus on the diagnostic or prognostic value of circulating Ang-1 and Ang-2.
Participant Demographics
94 Caucasian ICU patients, 56 males and 48 females, aged 59 years (21 to 69).
Statistical Information
P-Value
p<0.0001
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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