Induction of PNAd and N-acetylglucosamine 6-O-sulfotransferases in Mouse Collagen-Induced Arthritis
Author Information
Author(s): Yang Jiwei, Rosen Steven D, Bendele Philip, Hemmerich Stefan
Primary Institution: Thios Pharmaceuticals Inc.
Hypothesis
The study investigates the expression of PNAd and GlcNAc6ST-1 and -2 in a mouse model of collagen-induced arthritis.
Conclusion
The study shows that PNAd and the sulfotransferases GlcNAc6ST-1 and 2 are induced in mouse collagen-induced arthritis, suggesting potential therapeutic targets.
Supporting Evidence
- PNAd is expressed in the vasculature of arthritic synovium in mice immunized with collagen.
- GlcNAc6ST-2 transcripts were upregulated in diseased joints compared to control joints.
- GlcNAc6ST-2 protein was present in a Golgi-like pattern in PNAd+ vessels.
Takeaway
The study found that certain proteins involved in inflammation are more active in mice with arthritis, which could help in finding new treatments.
Methodology
The study used quantitative PCR and immunofluorescence to analyze the expression of PNAd and GlcNAc6ST-1 and -2 in synovial tissue from mice with collagen-induced arthritis.
Participant Demographics
18 male B10RIII mice, aged 8 ± 1 weeks.
Statistical Information
P-Value
p<0.0005
Statistical Significance
p<0.0005
Digital Object Identifier (DOI)
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