How Antioxidants Help Reduce Inflammation from Hyaluronan Fragments
Author Information
Author(s): Eberlein Michael, Scheibner Kara A, Black Katharine E, Collins Samuel L, Chan-Li Yee, Powell Jonathan D, Horton Maureen R
Primary Institution: Johns Hopkins University School of Medicine
Hypothesis
Can antioxidants like NAC and DMSO inhibit inflammatory gene expression induced by hyaluronan fragments in macrophages and epithelial cells?
Conclusion
The antioxidants NAC and DMSO can inhibit inflammatory gene expression induced by hyaluronan fragments, potentially helping to rebalance excessive inflammation caused by reactive oxygen species.
Supporting Evidence
- NAC and DMSO inhibit HA fragment-induced expression of TNF-α and KC protein in macrophages.
- NAC significantly inhibits HA fragment-induced TNF-α and KC in a dose-dependent manner.
- DMSO inhibits HA fragment-induced TNF-α by up to 87%.
- NAC inhibits HA fragment-induced IP-10 by 75–100%.
Takeaway
When our body gets hurt, it makes something called ROS that can cause more damage. This study found that two special helpers, NAC and DMSO, can stop this damage by calming down the bad reactions.
Methodology
The study evaluated the effects of H2O2, NAC, and DMSO on inflammatory gene expression in alveolar macrophages and epithelial cells.
Statistical Information
P-Value
p < 0.0023
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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