c-Myc's Role in Glioma Cancer Stem Cells
Author Information
Author(s): Wang Jialiang, Wang Hui, Li Zhizhong, Wu Qiulian, Lathia Justin D., McLendon Roger E., Hjelmeland Anita B., Rich Jeremy N.
Primary Institution: Duke University
Hypothesis
c-Myc may contribute to cancer stem cell biology in gliomas.
Conclusion
c-Myc is essential for the proliferation and survival of glioma cancer stem cells.
Supporting Evidence
- c-Myc is highly expressed in glioma cancer stem cells compared to non-stem glioma cells.
- Knockdown of c-Myc reduced proliferation and increased apoptosis in glioma cancer stem cells.
- Glioma cancer stem cells with decreased c-Myc levels failed to form tumors in mice.
Takeaway
c-Myc helps cancer stem cells in gliomas grow and survive, so stopping it could help treat brain tumors.
Methodology
The study used real-time PCR, immunoblotting, and flow cytometry to analyze c-Myc expression and its effects on glioma cancer stem cells.
Limitations
The study could not definitively determine if c-Myc is required for self-renewal due to limited viability of neurospheres formed by c-Myc depleted cells.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website