Identification of Nucleolin as New ErbB Receptors- Interacting Protein ErbB/Nucleolin Interactions
2008

Nucleolin's Role in ErbB Receptor Interactions

publication Evidence: moderate

Author Information

Author(s): Di Segni Ayelet, Farin Keren, Pinkas-Kramarski Ronit

Primary Institution: Tel-Aviv University

Hypothesis

Nucleolin interacts with ErbB receptors and affects their activation and signaling.

Conclusion

Nucleolin enhances ErbB receptor dimerization and activation, leading to increased cell growth.

Supporting Evidence

  • Nucleolin interacts with the cytoplasmic tail of ErbB4.
  • Nucleolin enhances ErbB receptor dimerization and phosphorylation.
  • Nucleolin reduces ligand-induced receptor degradation.
  • Nucleolin promotes anchorage-independent growth in cells.

Takeaway

Nucleolin helps certain proteins in our cells talk to each other better, which can make cells grow more, especially in cancer.

Methodology

The study used GST-ErbB4 fusion protein pull down assay and mass spectroscopic analysis to identify interactions.

Statistical Information

P-Value

p<0.001

Statistical Significance

p<0.001

Digital Object Identifier (DOI)

10.1371/journal.pone.0002310

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