Prostaglandin production in mouse mammary tumour cells confers invasive growth potential by inducing hepatocyte growth factor in stromal fibroblasts
1999
Prostaglandin E2 and Tumor Invasion in Mammary Cells
publication
Evidence: moderate
Author Information
Author(s): Matsumoto-Taniura N, Matsumoto K, Nakamura T
Primary Institution: Osaka University Medical School
Hypothesis
MMT mouse mammary tumor cells secrete a factor that induces hepatocyte growth factor (HGF) production in fibroblasts.
Conclusion
Prostaglandin E2 from MMT cells enhances HGF production in fibroblasts, which in turn promotes invasive growth in mammary tumor cells.
Supporting Evidence
- MMT cells do not produce HGF but induce its production in fibroblasts.
- Prostaglandin E2 was identified as the inducing factor for HGF.
- Indomethacin treatment abolished HGF-inducing activity in MMT-derived conditioned medium.
- HGF treatment stimulated cell growth and motility in MMT cells.
- HGF enhanced invasion of MMT cells through Matrigel by 15-fold.
- Co-cultivation with fibroblasts increased HGF levels and MMT cell invasion.
Takeaway
Mammary tumor cells can make a substance that helps nearby cells produce a growth factor, which makes the tumor cells grow and spread more.
Methodology
The study involved co-culturing MMT cells with human fibroblasts and measuring HGF levels and cell invasion.
Digital Object Identifier (DOI)
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