Nanoconjugated Vancomycin Reduces Oxidative Stress in Lymphocytes Infected with Staphylococcus aureus
Author Information
Author(s): Subhankari Prasad Chakraborty, Santanu Kar Mahapatra, Sumanta Kumar Sahu, Sabyasachi Das, Satyajit Tripathy, Sandeep Dash, Panchanan Pramanik, Somenath Roy
Primary Institution: Vidyasagar University, India
Hypothesis
The study aims to test the ameliorative role of nanoconjugated vancomycin against oxidative stress induced by Staphylococcus aureus infections in lymphocytes.
Conclusion
Nanoconjugated vancomycin effectively reduces oxidative stress and DNA fragmentation in lymphocytes infected with both vancomycin-sensitive and vancomycin-resistant Staphylococcus aureus.
Supporting Evidence
- Nanoconjugated vancomycin significantly decreased lipid peroxidation and protein oxidation in lymphocytes.
- DNA fragmentation was significantly reduced in lymphocytes treated with nanoconjugated vancomycin.
- Treatment with nanoconjugated vancomycin improved antioxidant enzyme activity in lymphocytes.
- Oxidized glutathione levels were significantly decreased in lymphocytes treated with nanoconjugated vancomycin.
Takeaway
This study shows that a special form of vancomycin can help protect immune cells from damage caused by a common germ.
Methodology
Swiss mice were infected with Staphylococcus aureus and treated with nanoconjugated vancomycin for 10 days, followed by biochemical assessments of oxidative stress markers.
Limitations
The study was conducted in mice, and results may not directly translate to humans.
Participant Demographics
Swiss male mice, 6–8 weeks old, weighing 20–25 g.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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