Production of a Single-Chain Antibody to ErbB-2 in Plants and Cell-Free Systems
Author Information
Author(s): Galeffi Patrizia, Lombardi Alessio, Pietraforte Immacolata, Novelli Flavia, Di Donato Monica, Sperandei Maria, Tornambé Andrea, Fraioli Rocco, Martayan Aline, Natali Pier Giorgio, Benevolo Maria, Mottolese Marcella, Ylera Francisco, Cantale Cristina, Giacomini Patrizio
Primary Institution: ENEA BIOTEC-GEN, CR Casaccia, Rome, Italy
Hypothesis
Can a single-chain antibody to ErbB-2 be effectively produced in plants and cell-free systems?
Conclusion
ScFv800E6 is a useful reagent for in vitro biochemical and immunodiagnostic applications in oncology, and a candidate for future in vivo studies.
Supporting Evidence
- ScFv800E6 was expressed and fully functional in all tested expression platforms.
- ScFv800E6 displayed a binding ability similar to that of the parental antibody.
- ScFv800E6 was resistant to standard chemical radiolabeling procedures.
- ScFv800E6 was found compatible with routine reagents for immunohistochemical staining.
Takeaway
Scientists created a special type of antibody in plants that can help detect cancer. This antibody works well and could be used in future treatments.
Methodology
The study involved producing a single-chain antibody (ScFv800E6) in bacteria, transgenic tobacco plants, and a cell-free system, followed by testing its binding ability and functionality.
Limitations
The yield of ScFv800E6 from stable transgenic plants was low compared to other systems, and there may be issues with transgene silencing affecting expression.
Digital Object Identifier (DOI)
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