Effects of Acidosis on Oxygen Release from Stored Blood
Author Information
Author(s): Helge Opdahl, Tæwje A Strømme, Lise Jørgensen, Livia Bajelan, Hans E Heier
Primary Institution: Oslo University Hospital
Hypothesis
To what extent can acidosis compensate for the storage-induced leftward shift in erythrocyte concentrates?
Conclusion
The rightward shift of the HbO2 curve due to acidosis is well maintained in stored erythrocytes, and a moderate pH decrease offsets the storage-induced increased HbO2 affinity.
Supporting Evidence
- Acidification right-shifted the HbO2 curve, making it similar to fresh blood after 2.5 weeks of storage.
- The leftward shift of the HbO2 curve was established after 1 week of storage.
- Consumable O2 decreased continuously with increasing acidosis at low PO2 levels.
Takeaway
When blood is stored, it holds onto oxygen more tightly, but if the blood becomes more acidic, it can release that oxygen better, which is important for patients who need blood transfusions.
Methodology
Blood from 18 healthy donors was processed and stored as erythrocyte concentrates, and the HbO2 curve was measured at various pH levels over a storage period of up to 35 days.
Limitations
The study was limited to a specific storage method and may not apply to all blood storage techniques.
Participant Demographics
Eighteen healthy persons from the donor pool of Oslo University Hospital Blood Bank.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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