Expression of human milk fat globulin proteins in cells of haemopoietic origin
2000
Expression of human milk fat globulin proteins in blood cells
publication
Author Information
Author(s): Krüger W, Lohner R, Jung R, Kröger N, Zander A R
Primary Institution: University-Hospital Eppendorf
Hypothesis
The study investigates the expression of mammary epithelium related antigens in haemopoietic cells.
Conclusion
The findings suggest that lineage-specific gene expression is not valid at the molecular level.
Supporting Evidence
- Both mRNA molecules were amplified by rtPCR from the samples.
- Peptide-specific antibodies were raised in rabbits and used for Western-blot analysis.
- Antibody P717 showed strong staining in myeloid cell lines suggesting lactadherin's role in leukocyte function.
Takeaway
The study found that certain proteins related to breast tissue are also present in blood cells, which changes how we think about gene expression in different cell types.
Methodology
The study used Northern-hybridization, rtPCR, Western-blot analysis, and immunocytochemical studies to analyze gene expression.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website