Combining Trichosanthin and Benserazide to Fight Cancer
Author Information
Author(s): Zhou Yan, Ran Maoxin, Shan Wenying, Wang Kaifang, Sha Ou, Tam Kin Yip
Primary Institution: University of Macau
Hypothesis
Can targeting hexokinase 2 enhance the anticancer efficacy of trichosanthin in HeLa and SCC25 cell models?
Conclusion
The combination of trichosanthin and benserazide significantly enhances anticancer effects by modulating glycolysis and inducing apoptosis in cancer cells.
Supporting Evidence
- The combination of TCS and Benz showed synergistic anticancer effects in both HeLa and SCC25 cell models.
- Targeting HK2 led to reduced lactate production and increased ROS accumulation.
- The combination treatment significantly suppressed tumor growth in SCC25 cell xenograft mouse model.
- Cell cycle analysis indicated G0/G1 phase arrest in both HeLa and SCC25 cells.
- Combination treatment increased intracellular ROS and triggered apoptosis in cancer cells.
Takeaway
This study shows that mixing two medicines can help fight cancer better than using them alone.
Methodology
The study used MTT, colony-formation, and cell cycle assays, along with Seahorse analysis, western blotting, flow cytometry, and RNA sequencing to assess the effects of trichosanthin and benserazide on cancer cells.
Limitations
The study primarily focuses on in vitro and in vivo models, which may not fully replicate human responses.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website