AtRTPrimer: A Database for Arabidopsis RT-PCR Primer Pairs
Author Information
Author(s): Han Sangjo, Kim Dongsup
Primary Institution: Korea Advanced Institute of Science and Technology
Hypothesis
To construct a genome-wide primer-pair database for Arabidopsis that provides specific and reliable RT-PCR primer pairs.
Conclusion
AtRTPrimer successfully registered approximately 97% of the genes investigated, providing a large number of reliable primer pairs for RT-PCR experiments.
Supporting Evidence
- 97% of the 28,952 genes were registered in AtRTPrimer.
- The database provides reliable primer pairs for various RT-PCR experiments.
- AtRTPrimer allows researchers to filter primer pairs based on specific needs.
Takeaway
The AtRTPrimer database helps scientists find the right tools to study genes in plants, making it easier to do experiments.
Methodology
The database was constructed by evaluating primer homogeneity, specificity, and potential amplicons from genomic DNA and similar cDNAs.
Limitations
The database may not predict amplicons from unknown alternative splicing forms.
Digital Object Identifier (DOI)
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