Breast Cells with CD44+/CD24- Phenotype and EMT Genes
Author Information
Author(s): Bhat-Nakshatri Poornima, Appaiah Hitesh, Ballas Christopher, Pick-Franke Patricia, Goulet Robert Jr, Badve Sunil, Srour Edward F, Nakshatri Harikrishna
Primary Institution: Indiana University School of Medicine
Hypothesis
Only a subset of genes associated with EMT generates CD44+/CD24- cells.
Conclusion
EMT-mediated generation of CD44+/CD24- or CD44+/CD24+ cells depends on the genes that induce or are associated with EMT.
Supporting Evidence
- Two thousand thirty five genes were differentially expressed between the CD44+/CD24- and CD44-/CD24+ subpopulations.
- Thirty-two EMT-associated genes including SLUG and Gli-2 were expressed at higher levels in CD44+/CD24- cells.
- Treatment with tumor necrosis factor induced a subpopulation of CD44+/CD24- cells.
Takeaway
Some genes can change breast cells to a type that might help cancer grow, and one of those genes is called SLUG.
Methodology
MCF-10A breast epithelial cells were used to identify differentially expressed genes between CD44+/CD24- and CD44-/CD24+ cells, with flow cytometry and mammosphere assays to assess properties.
Limitations
The study primarily focuses on a specific cell line and may not fully represent all breast cancer types.
Statistical Information
P-Value
<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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