Protein Profile of Gasterophilus intestinalis Larvae and Horse Immune Response
Author Information
Author(s): Roelfstra Liselore, Deeg Cornelia A, Hauck Stefanie M, Buse Christina, Membrez Mathieu, Betschart Bruno, Pfister Kurt
Primary Institution: Institute of Biology, University of Neuchâtel, Switzerland
Hypothesis
The study aims to analyze the protein content of Gasterophilus intestinalis larvae and characterize the immune response of horses.
Conclusion
The study provides insights into the interaction between Gasterophilus intestinalis and horses, highlighting the immunogenic differences between larval stages.
Supporting Evidence
- The proteomic profile of larval stages L2 and L3 showed distinct migration patterns.
- L2 larvae elicited a stronger immune response compared to L3 larvae.
- Fifteen proteins were identified in L2 and ten in L3 using mass spectrometry.
Takeaway
This study looks at how certain larvae affect horses and how the horses' immune systems react to them.
Methodology
The study involved collecting larvae from horses, preparing crude extracts, and analyzing protein profiles using one and two-dimensional electrophoresis and mass spectrometry.
Potential Biases
The immune response in mice may not accurately reflect the natural immune response in horses due to the artificial immunization method used.
Limitations
The study did not evaluate the risk of cross-reactivity with other intestinal parasites present in the horses.
Participant Demographics
The study involved horses from two farms in the Swiss Jura region.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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