Biologically active fibroblast growth factor 1 tagged with various epitopes
2008
Study of Tagged Fibroblast Growth Factor 1
publication
Evidence: moderate
Author Information
Author(s): Asada Masahiro, Honda Emi, Imamura Toru
Primary Institution: National Institute of Advanced Industrial Science and Technology (AIST)
Hypothesis
Does tagging fibroblast growth factor 1 (FGF1) with various epitopes affect its biological activity?
Conclusion
Tagged FGF1s maintain their biological activity and affinity for heparin similar to the native form.
Supporting Evidence
- Tagged FGF1s showed similar affinities for heparin as the native form.
- The mitogenic activity of tagged FGF1s was indistinguishable from that of native FGF1.
- Statistical analysis revealed no significant difference in activity between tagged and native FGF1.
Takeaway
Scientists added tags to a protein called FGF1 to see if it would still work the same way, and it did!
Methodology
E. coli was used to express tagged FGF1 proteins, which were then analyzed for their affinity to heparin and mitogenic activity.
Digital Object Identifier (DOI)
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