Critical Period of DNA Methylation in Male Germ Cell Development
Author Information
Author(s): Niles Kirsten M., Chan Donovan, La Salle Sophie, Oakes Christopher C., Trasler Jacquetta M.
Primary Institution: McGill University and Research Institute at The Montreal Children's Hospital
Hypothesis
The study investigates the timing and dynamics of DNA methylation acquisition in male germ cells during prenatal development.
Conclusion
The critical period for DNA methylation programming in male germline progenitor cells occurs prenatally, and external factors can influence this process.
Supporting Evidence
- DNA methylation patterns are established during gametogenesis.
- Most DNA methylation acquisition occurs before the formation of type A spermatogonia.
- Intergenic sequences acquire methylation primarily during the prenatal period.
Takeaway
This study shows that male baby cells start to change their DNA in important ways before they are born, and things like mom's diet can affect this.
Methodology
DNA methylation was analyzed using restriction landmark genomic scanning and quantitative PCR on germ cells isolated from mice.
Limitations
The study is limited by the difficulty in isolating sufficient numbers of pure germ cells.
Participant Demographics
Wildtype and DNMT3L-deficient mice were used in the study.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.001
Digital Object Identifier (DOI)
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