The HIRA Complex Subunit Hip3 Plays Important Roles in the Silencing of Meiosis-Specific Genes in Schizosaccharomyces pombe
2011
Important Roles of Hip3 in Gene Silencing
publication
Evidence: moderate
Author Information
Author(s): Mizuki Fumitaka, Tanaka Aki, Ohkuma Yutaka, Yoshiaki
Primary Institution: University of Toyama, Toyama, Japan
Hypothesis
Hip3 plays important roles in the control of histone modifications at meiosis-specific gene loci and induces their transcriptional repression.
Conclusion
Hip3 is essential for the transcriptional repression of meiosis-specific genes in Schizosaccharomyces pombe.
Supporting Evidence
- Hip3 is a subunit of the HIRA complex, which is involved in histone modification.
- The hip3-1 mutant shows increased expression of meiosis-specific genes.
- ChIP assays revealed altered histone modifications in hip3-1 cells.
- Hip3 is necessary for the transcriptional repression of the SPCC663.14c gene.
Takeaway
Hip3 is a protein that helps turn off certain genes during cell division, and when it doesn't work right, those genes can turn on when they shouldn't.
Methodology
The study involved isolating a hip3 mutant and conducting ChIP assays to analyze histone modifications and transcriptional activity.
Digital Object Identifier (DOI)
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