Functional Role of Glutamine 28 and Arginine 39 in Double Stranded RNA Cleavage by Human Pancreatic Ribonuclease
2011
Role of Glutamine 28 and Arginine 39 in RNA Cleavage by Human Pancreatic Ribonuclease
publication
Evidence: moderate
Author Information
Author(s): Rehman Md. Tabish, Dey Punyatirtha, Hassan Md. Imtaiyaz, Ahmad Faizan, Batra Janendra K.
Primary Institution: National Institute of Immunology, New Delhi, India
Hypothesis
What is the role of glutamine 28 and arginine 39 in the activity of human pancreatic ribonuclease on double stranded RNA?
Conclusion
Glutamine 28 and arginine 39 are important for the cleavage of double stranded RNA by human pancreatic ribonuclease.
Supporting Evidence
- Mutations in glutamine 28 and arginine 39 significantly reduced the enzyme's activity on double stranded RNA.
- The study demonstrated that the catalytic efficiency of ribonuclease variants was affected by these mutations.
- Far-UV CD-spectral analysis showed that most variants maintained similar structures to the wild type ribonuclease.
Takeaway
This study found that two specific parts of a protein help it cut up a type of RNA that can be harmful to our bodies.
Methodology
The study involved creating nine variants of the human pancreatic ribonuclease and analyzing their activity on double stranded RNA and single stranded RNA.
Digital Object Identifier (DOI)
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