Piper sarmentosum and its effects on endothelial cells
Author Information
Author(s): Ugusman Azizah, Zakaria Zaiton, Hui Chua Kien, Megat Mohd Nordin Nor Anita
Primary Institution: Department of Physiology, Faculty of Medicine, Universiti Kebangsaan Malaysia Medical Centre
Hypothesis
Does the aqueous extract of Piper sarmentosum affect gene expression related to oxidative stress in human umbilical vein endothelial cells?
Conclusion
Piper sarmentosum reduces the expression of ICAM-1 and Nox4 while increasing antioxidant enzyme levels in endothelial cells.
Supporting Evidence
- HUVECs treated with H2O2 showed a significant increase in ICAM-1 expression.
- AEPS treatment reduced ICAM-1 and Nox4 expression in HUVECs.
- AEPS increased the expression of antioxidant enzymes SOD1, CAT, and GPx.
Takeaway
Piper sarmentosum can help protect blood vessels by lowering harmful proteins and boosting the body's defenses against stress.
Methodology
Human umbilical vein endothelial cells were treated with hydrogen peroxide and Piper sarmentosum extract, followed by gene expression analysis using qPCR.
Limitations
The study could not determine the specific components of Piper sarmentosum responsible for the observed effects.
Participant Demographics
Human umbilical cord veins were used for cell culture.
Statistical Information
P-Value
p < 0.01
Statistical Significance
p < 0.05
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website