Pig Multi-Tissue cDNA Library and Microarray Development
Author Information
Author(s): Bonnet Agnès, Iannuccelli Eddie, Hugot Karine, Benne Francis, Bonaldo Maria F, Soares Marcelo B, Hatey François, Tosser-Klopp Gwenola
Primary Institution: Institut National de la Recherche Agronomique (INRA)
Hypothesis
The study aims to create a high-throughput cDNA sequencing program and a microarray for transcriptome analysis in pigs.
Conclusion
A pig multi-tissue cDNA library was constructed and a 9K cDNA microarray designed, contributing valuable resources for transcriptome analysis.
Supporting Evidence
- A total of 24,449 high-quality sequences were released in the EMBL database.
- The assembly resulted in 40,786 contigs and 54,653 singletons.
- The microarray allows high sensitivity transcriptome analyses in pigs.
Takeaway
Researchers made a special library of pig genes from many different tissues to help understand how these genes work together.
Methodology
The study involved constructing a multi-tissue cDNA library from 38 tissues, normalising and subtracting to reduce redundancy, and sequencing to generate expressed sequence tags.
Limitations
The study may have limitations related to the representation of specific tissues and potential contamination in the cDNA library.
Participant Demographics
Tissue samples were taken from Meishan and Large White pigs at different developmental stages and physiological conditions.
Digital Object Identifier (DOI)
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