Automated colorimetric determination of recombinant fungal laccase activity in fermentation samples using syringaldazine as chromogenic substrate
1998
Automated Method for Measuring Fungal Laccase Activity
publication
Evidence: moderate
Author Information
Author(s): K. A. Holm, D. M. Nielsen, J. Eriksen
Primary Institution: Novo Nordisk Research Institute
Hypothesis
An automated method can accurately determine the activity of recombinant M. thermophila laccase using syringaldazine as a substrate.
Conclusion
The developed automated method provides a sensitive and precise way to measure laccase activity.
Supporting Evidence
- The method showed good precision with a repeatability of 1.9% and reproducibility of 3.1%.
- The limit of detection (LOD) was determined to be 0.0002 Um1-1.
- The limit of quantitation (LOQ) was determined to be 0.010 Um1-1.
- The Michaelis-Menten constant (Km) was found to be 22 µM for syringaldazine.
Takeaway
Scientists created a fast and easy way to measure how well a special enzyme works using a color change.
Methodology
The method involved using an automated Cobas Fara analyzer to measure the enzyme activity based on the color change of syringaldazine.
Limitations
The method's accuracy can be affected by various factors such as temperature and pH.
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