Exposure to Sublethal Doses of Fipronil and Thiacloprid Highly Increases Mortality of Honeybees Previously Infected by Nosema ceranae
2011

Impact of Nosema Infection and Insecticides on Honeybee Mortality

Sample size: 2000 publication 10 minutes Evidence: high

Author Information

Author(s): Vidau Cyril, Diogon Marie, Aufauvre Julie, Fontbonne Régis, Viguès Bernard, Brunet Jean-Luc, Texier Catherine, Biron David G., Blot Nicolas, El Alaoui Hicham, Belzunces Luc P., Delbac Frédéric

Primary Institution: Clermont Université, Université Blaise Pascal, Laboratoire Microorganismes: Génome et Environnement

Hypothesis

Can Nosema ceranae infection increase honeybee mortality when exposed to sublethal doses of fipronil and thiacloprid?

Conclusion

Nosema ceranae-infected honeybees showed significantly higher mortality rates when exposed to sublethal doses of fipronil and thiacloprid compared to uninfected honeybees.

Supporting Evidence

  • Nosema ceranae infection significantly increased honeybee mortality when exposed to insecticides.
  • Fipronil exposure reduced spore production in infected bees, while thiacloprid increased it.
  • GST activity was significantly higher in infected honeybees, indicating a response to infection.

Takeaway

If honeybees get sick from a tiny bug called Nosema and then eat some bug spray, they are much more likely to die than healthy bees that eat the same spray.

Methodology

Honeybees were divided into experimental groups, some infected with Nosema ceranae and others exposed to insecticides, with daily monitoring of mortality and insecticide consumption.

Potential Biases

Potential bias in the selection of honeybee colonies and environmental conditions during the experiment.

Limitations

The study did not explore the long-term effects of insecticide exposure on honeybee health beyond the 20-day observation period.

Participant Demographics

Honeybees from three Buckfast colonies, approximately 2000 bees used in total.

Statistical Information

P-Value

p<0.001

Statistical Significance

p<0.001

Digital Object Identifier (DOI)

10.1371/journal.pone.0021550

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