Fast Analysis of Microbial Rhamnolipids Using ATR-FTIR Spectroscopy
Author Information
Author(s): Frank Leitermann, Christoph Syldatk, Rudolf Hausmann
Primary Institution: Research University Karlsruhe, Institute of Engineering in Life Sciences, Section of Technical Biology
Hypothesis
Can ATR-FTIR spectroscopy provide a rapid and accurate method for quantifying microbial rhamnolipids in cultivation broths?
Conclusion
ATR-FTIR is suitable for the rapid analysis of rhamnolipids in biotechnological processes with good reproducibility and sufficient accuracy.
Supporting Evidence
- ATR-FTIR can quantify rhamnolipids in 20 minutes compared to HPLC which takes about 24 hours.
- The method showed good reproducibility with minimal diversities in spectra.
- Cross-validation of calibration models yielded high determination coefficients.
Takeaway
Scientists found a quick way to measure a special substance made by bacteria, which usually takes a long time to analyze.
Methodology
The study used ATR-FTIR spectroscopy to analyze rhamnolipids from cultivation broths, comparing results with HPLC for validation.
Limitations
The study may be limited by the complexity of the analyte-matrix in real cultivation samples.
Digital Object Identifier (DOI)
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