Mitotic phosphorylation activates hepatoma-derived growth factor as a mitogen
2011
Phosphorylation of HDGF is important for cell growth
Sample size: 3
publication
Evidence: moderate
Author Information
Author(s): Everett Allen D, Yang Jun, Rahman Monzur, Dulloor Pratima, Brautigan David L
Primary Institution: Johns Hopkins University
Hypothesis
Does phosphorylation of HDGF affect its function as a mitogen?
Conclusion
Phosphorylation of S103 is necessary for HDGF's function as a mitogen and regulates cell proliferation.
Supporting Evidence
- HDGF was found to be phosphorylated in primary mouse aortic vascular smooth muscle cells.
- Phosphorylation of S103 was shown to be necessary for HDGF's mitogenic activity.
- Cells expressing HDGF-S103A did not show increased cell proliferation.
Takeaway
HDGF helps cells grow, and it needs to be changed a little (phosphorylated) during cell division to do its job.
Methodology
The study involved cell culture, transfections, and FACS analysis to assess the effects of HDGF phosphorylation on cell proliferation.
Statistical Information
P-Value
0.037
Statistical Significance
p = 0.037
Digital Object Identifier (DOI)
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