Dibutyltin Disrupts Glucocorticoid Receptor Function
Author Information
Author(s): Gumy Christel, Chandsawangbhuwana Charlie, Dzyakanchuk Anna A., Kratschmar Denise V., Baker Michael E., Odermatt Alex
Primary Institution: University of Basel
Hypothesis
DBT affects one or more glucocorticoid responses because this steroid has important actions on the immune system.
Conclusion
DBT inhibits ligand binding to GR and subsequent activation of the receptor, potentially disturbing metabolic functions and immune system modulation.
Supporting Evidence
- DBT inhibits ligand binding to GR and its transcriptional activity.
- DBT disrupts glucocorticoid-induced expression of key metabolic enzymes.
- DBT abrogates the glucocorticoid-mediated suppression of pro-inflammatory cytokines.
- DBT alters the conformation of the glucocorticoid receptor, affecting its function.
Takeaway
Dibutyltin, a chemical found in some plastics, can stop a key part of the immune system from working properly, which might make people more sick.
Methodology
The study used HEK-293 cells, rat H4IIE hepatoma cells, and human macrophages to investigate the effects of DBT on GR function through ligand binding assays and gene expression analysis.
Limitations
The study primarily focused on in vitro models, which may not fully replicate in vivo conditions.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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