Effects of Nelfinavir on Notch 4 Pathway in Cerebral Endothelial Cells
Author Information
Author(s): Grigorian Aline, Hurford Rosemary, Chao Ying, Patrick Christina, Langford T Dianne
Primary Institution: University of California San Diego
Hypothesis
Notch expression and processing may become vulnerable to dysregulation in cerebral endothelial cells during exposure to HIV protease inhibitors.
Conclusion
Nelfinavir affects Notch 4 processing, leading to increased expression of pro-angiogenic genes in cerebral endothelial cells.
Supporting Evidence
- Nelfinavir increased Notch 4 processing and nuclear localization of its intracellular domain.
- Vitamin E pre-treatment prevented Nelfinavir-induced reactive oxygen species generation.
- Nelfinavir did not inhibit angiogenic capacity in cerebral endothelial cells.
Takeaway
Nelfinavir, a drug used to treat HIV, changes how certain proteins work in brain blood vessel cells, which might help those cells grow and heal.
Methodology
Cerebral endothelial cells were treated with HIV protease inhibitors and analyzed for cell viability, reactive oxygen species generation, and Notch signaling.
Limitations
The study primarily focuses on Notch 4 and does not address other potential pathways affected by HIV protease inhibitors.
Statistical Information
P-Value
p<0.001
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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