Cholinergic Neuromodulation Changes Phase Response Curve Shape and Type in Cortical Pyramidal Neurons
2008

Cholinergic Modulation Changes Neuron Spike Response

Sample size: 9 publication Evidence: moderate

Author Information

Author(s): Stiefel Klaus M., Gutkin Boris S., Sejnowski Terrence J.

Primary Institution: Howard Hughes Medical Institute, The Salk Institute for Biological Studies

Hypothesis

Cholinergic neuromodulation can switch the phase response curve type in cortical pyramidal neurons.

Conclusion

Cholinergic action can change the phase response curve of cortical pyramidal neurons from type II to type I, affecting their spike generation dynamics.

Supporting Evidence

  • Cholinergic modulation switched the PRC from type II to type I in all type II neurons tested.
  • No type I neurons switched to type II after cholinergic application.
  • The study provides the first evidence of a qualitative switch in the dynamics of spike generation in neurons.

Takeaway

This study shows that a chemical in the brain can change how brain cells fire signals, which might help them work together better.

Methodology

Electrophysiological recordings were made from layer II/III pyramidal neurons in mouse visual cortex slices to determine their phase response curves before and after cholinergic modulation.

Limitations

Variability in neuronal responses and the inability to ascertain significance across the cell population due to strong variability.

Participant Demographics

Mice (B6D21/Hsd B6, age P28 to P35) were used for the experiments.

Statistical Information

P-Value

p<0.01

Statistical Significance

p<0.01

Digital Object Identifier (DOI)

10.1371/journal.pone.0003947

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