Structural and Sequence Analysis of Imelysin-Like Proteins Implicated in Bacterial Iron Uptake
2011

Structure and Function of Imelysin-Like Proteins in Bacterial Iron Uptake

publication 10 minutes Evidence: moderate

Author Information

Author(s): Xu Qingping, Rawlings Neil D., Farr Carol L., Chiu Hsiu-Ju, Grant Joanna C., Jaroszewski Lukasz, Klock Heath E., Knuth Mark W., Miller Mitchell D., Weekes Dana, Elsliger Marc-André, Deacon Ashley M., Godzik Adam, Lesley Scott A., Wilson Ian A.

Primary Institution: Joint Center for Structural Genomics, La Jolla, California, United States of America

Hypothesis

Imelysin-like proteins have evolved from a common ancestor and likely have a conserved function in iron uptake.

Conclusion

The study reveals that imelysin-like proteins are not peptidases and have distinct structural features that suggest a role in iron uptake.

Supporting Evidence

  • Imelysin-like proteins define a superfamily of bacterial proteins likely involved in iron uptake.
  • The study determined the first crystal structures of two imelysin-like proteins.
  • Both structures are highly similar despite low sequence similarity.
  • Imelysin-like proteins are not peptidases and have evolved distinct structural features.

Takeaway

Scientists studied proteins that help bacteria take in iron, finding that these proteins have unique shapes and likely work together in this process.

Methodology

The study involved determining the crystal structures of two imelysin-like proteins using high-throughput structural genomics methods.

Limitations

The biochemical functions and substrate specificity of imelysin-like proteins remain poorly understood.

Digital Object Identifier (DOI)

10.1371/journal.pone.0021875

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