Modeling Stem Cell Movement During Ischemia
Author Information
Author(s): Bailey Alexander M., Lawrence Michael B., Shang Hulan, Katz Adam J., Peirce Shayn M.
Primary Institution: University of Virginia
Hypothesis
A better understanding of the trafficking of therapeutic hASCs through the microcirculation is needed to enhance their homing to sites of injury.
Conclusion
The study found that a small percentage of hASCs can roll on P-selectin, which may limit their ability to home effectively to ischemic tissues.
Supporting Evidence
- Model simulations predicted that hASCs require an unknown selectin-binding molecule for effective extravasation.
- In vitro experiments confirmed that a subpopulation of hASCs can roll on immobilized P-selectin.
- The model reasonably reproduced key aspects of ischemia and trafficking behavior.
- Simulations indicated that selectin interactions are critical for hASC trafficking.
- Only about 1% of hASCs were observed to roll on P-selectin in vitro.
Takeaway
Scientists created a computer model to understand how stem cells move in the body during injuries, and they found that only a tiny bit of these cells can stick to the blood vessels to help heal.
Methodology
The study used an agent-based model to simulate hASC trafficking during acute skeletal muscle ischemia, incorporating over 150 literature-based rules.
Potential Biases
The model's predictions may not fully capture the complexity of in vivo conditions.
Limitations
The model does not account for all potential mediators of circulating cell trafficking and is based on simplified assumptions.
Participant Demographics
The study involved human adipose-derived stromal cells isolated from 8 patients (6 female, 2 unknown) aged 20-60.
Statistical Information
P-Value
p<0.05
Statistical Significance
p<0.05
Digital Object Identifier (DOI)
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