How Lactobacillus plantarum Affects Human Intestinal Mucosa
Author Information
Author(s): Freddy J Troost, Peter van Baarlen, Patrick Lindsey, André Kodde, Willem M de Vos, Michiel Kleerebezem, Robert-Jan M Brummer
Primary Institution: Maastricht University
Hypothesis
This study aimed to identify the acute, time-dependent responses of intestinal mucosa to commensal Lactobacillus plantarum WCFS1 in vivo.
Conclusion
The study showed that intestinal exposure to L. plantarum WCFS1 induced consistent, time-dependent transcriptional responses in healthy intestinal mucosa.
Supporting Evidence
- One- and six-hour exposure to L. plantarum WCFS1 induced differential expression of 669 and 424 gene reporters, respectively.
- Short-term exposure inhibited fatty acid metabolism and cell cycle progression, while prolonged exposure upregulated genes involved in lipid metabolism and cellular growth.
- Proteome analysis showed differential expression of several proteins, including microsomal triglyceride transfer protein.
Takeaway
When people eat Lactobacillus plantarum, their intestines react in different ways over time, first slowing down and then speeding up growth and healing.
Methodology
The study involved two placebo-controlled human intervention studies where healthy volunteers received intraduodenal infusions of L. plantarum WCFS1 for 1 hour and 6 hours, respectively, with tissue sampling performed via gastroduodenoscopy.
Limitations
The study was limited to healthy volunteers and may not generalize to individuals with gastrointestinal conditions.
Participant Demographics
Eight healthy non-smoking volunteers aged 24 ± 4 years for study 1 and seven healthy non-smoking volunteers aged 28 ± 6 years for study 2.
Digital Object Identifier (DOI)
Want to read the original?
Access the complete publication on the publisher's website