The priB Gene of Klebsiella pneumoniae Encodes a 104-Amino Acid Protein That Is Similar in Structure and Function to Escherichia coli PriB
2011
Klebsiella pneumoniae PriB Protein Similar to E. coli PriB
publication
Evidence: moderate
Author Information
Author(s): Linda Berg, Matthew E. Lopper
Primary Institution: University of Dayton
Hypothesis
The priB gene of Klebsiella pneumoniae encodes a 104-amino acid PriB protein similar in structure and function to E. coli PriB.
Conclusion
The PriB homolog of Klebsiella pneumoniae is similar in structure and function to that of E. coli.
Supporting Evidence
- K. pneumoniae PriB protein is proposed to be 104 amino acids long, similar to E. coli PriB.
- K. pneumoniae PriB binds to single-stranded DNA with an affinity similar to that of E. coli PriB.
- The study cloned and purified the K. pneumoniae PriB protein for analysis.
Takeaway
Klebsiella pneumoniae has a protein called PriB that works like the PriB protein in E. coli, helping with DNA replication.
Methodology
The study involved sequence analysis, cloning, purification of the PriB protein, and DNA binding activity assays.
Digital Object Identifier (DOI)
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